Plasmid_Backbone

Part:BBa_K4840004:Design

Designed by: Yueyu Zhang   Group: iGEM23_Jiangsu-United   (2023-09-18)


RNAi-Ready pSIREN-RetroQ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 3176
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 1567
    Illegal BamHI site found at 1825
    Illegal XhoI site found at 3027
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal AgeI site found at 1959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 623
    Illegal BsaI site found at 644
    Illegal BsaI site found at 3505
    Illegal BsaI site found at 3526
    Illegal BsaI.rc site found at 710
    Illegal BsaI.rc site found at 1311
    Illegal BsaI.rc site found at 2846
    Illegal BsaI.rc site found at 3593
    Illegal BsaI.rc site found at 5358
    Illegal SapI site found at 4275


Design Notes

NO


Source

NO

References